Hi Dany,
If your spots have different x,y locations, then a CellSpace image should work for this purpose. You can also create different images for different depths by limiting the selection timings. That is, to create an image of the upmost layer, choose only the first 5 s in your selections. Then create a new group, and select the second 5 s etc. This way, each image will be of a different depth.
If that doesn't answer your question, please just let me know.
Best regards,
Bence